Invitrogen Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit, 10 x 96 Tests

Invitrogen Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit, 10 x 96 Tests

The Dehydroepiandrosterone Sulfate (DHEA-S) ELISA quantitates DHEA-Se in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.

Principle of the method

The Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Dehydroepiandrosterone sulfate (DHEA-S) in serum, plasma, urine, and saliva samples, and in fecal extracts. The kit will also quantitatively measure DHEA-S present in tissue culture media samples. A DHEA-S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A DHEA-S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to DHEA-S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound DHEA-S-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Features  

• Homogenous (no wash): No    
• Interassay CV: 8.4%   
• Intraassay CV: 7%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Pre-coated 96 well plate
• Standard
• Assay Diluent concentrate
• HRP-Conjugated Detection Antibody
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers