Invitrogen™ DetectaGene™ Green CMFDG lacZ Gene Expression Kit
On demand
Order number :
D2920
Invitrogen™ DetectaGene™ Green CMFDG lacZ Gene Expression Kit
The DetectaGene™ Green lacZ Gene Expression Kit includes a fluorescein-based β-galactose derivative that has been chemically modified to include a mildly thiol-reactive chloromethyl group. Once inside a cell, the DetectaGene™ substrate undergoes two reactions: 1) its two galactose moieties are cleaved by intracellular β-galactosidase and 2) either simultaneously or sequentially, its chloromethyl moiety reacts with glutathione and possibly other intracellular thiols to form a membrane-impermeant, peptide-fluorescent dye adduct.
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