Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit, 100 Reactions

Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit, 100 Reactions

The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.

This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates. When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by Green Renilla Luc is considerably greater than signal from either native firefly or Renilla luciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression.

For use woth: Luminometer (Microplate)

Features

• Sensitive—greater sensitivity allows utilization of smaller numbers of cells
• Cost effective—highest sensitivity assays result in decreased reagent consumption
• Time-saving—assays require minimal sample handling
• Automation-friendly—amenable to high throughput screens
• Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
• Safe—allows one to perform non-radioactive assays

Contents

• Renilla Flash Assay Buffer, 5 mL, store at 4°C
• Coelenterazine (100X), 50 µL, store at -80°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature