Invitrogen DKK1 Human ELISA Kit, 5 x 96 Tests

Invitrogen DKK1 Human ELISA Kit, 5 x 96 Tests

The Human DKK1 ELISA quantitates Hu DKK1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DKK1.

Principle of the method

The Human DKK1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents    

• Human DKK-1 Antibody Coated wells, 96-well plate, 1 plate 
• Human DKK-1 Biotin Conjugate, 2 vials 
• Human DKK-1 Standard, recombinant human DKK-1, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent D (5X), 15 mL 
• Assay Diluent B (5X), 15 mL 
• Streptavidin-HRP (700X), 0.2 mL 
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2