Invitrogen DNA Damage Competitive ELISA Kit, 10 x 96 Tests

Invitrogen DNA Damage Competitive ELISA Kit, 10 x 96 Tests

The DNA Damage ELISA quantitates PGFM in serum, plasma, saliva, urine, digested DNA, fecal extracts, or cell culture medium.

Principle of the method

The DNA Damage Competitive ELISA research-use-only kit detects all three oxidized guanine species; 8-hydroxy-2'- deoxyguanosine from DNA, 8-hydroxyguanosine from RNA, and 8-hydroxyguanine from digested DNA from DNA or RNA. These species may be present in serum, plasma, saliva, urine, dried fecal samples, and tissue culture media samples. An 8-hydroxy-2'-deoxyguanosine (8-OHdG) stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An 8-hydroxyguanosine conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a peroxidase-labeled mouse monoclonal antibody to 8-hydroxy-2'-deoxyguanosine to each well. After a 1-hour incubation the plate is washed and substrate added. The substrate reacts with the peroxidase labeled antibody that has reacted with the bound conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Features  

• Homogenous (no wash): No    
• Interassay CV: 9.9%
• Intraassay CV: 9%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Pre-coated 96 well plate
• Standard
• Assay Diluent concentrate
• HRP-Conjugated Detection Antibody
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers