Invitrogen DNA Damage Competitive ELISA Kit, 96 Tests

Invitrogen DNA Damage Competitive ELISA Kit, 96 Tests

The DNA Damage ELISA quantitates PGFM in serum, plasma, saliva, urine, digested DNA, fecal extracts, or cell culture medium.

Principle of the method

The DNA Damage Competitive ELISA research-use-only kit detects all three oxidized guanine species; 8-hydroxy-2'- deoxyguanosine from DNA, 8-hydroxyguanosine from RNA, and 8-hydroxyguanine from digested DNA from DNA or RNA. These species may be present in serum, plasma, saliva, urine, dried fecal samples, and tissue culture media samples. An 8-hydroxy-2'-deoxyguanosine (8-OHdG) stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An 8-hydroxyguanosine conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a peroxidase-labeled mouse monoclonal antibody to 8-hydroxy-2'-deoxyguanosine to each well. After a 1-hour incubation the plate is washed and substrate added. The substrate reacts with the peroxidase labeled antibody that has reacted with the bound conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Features  

• Homogenous (no wash): No    
• Interassay CV: 9.9%
• Intraassay CV: 9%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Pre-coated 96 well plate
• Standard
• Assay Diluent concentrate
• HRP-Conjugated Detection Antibody
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers