Invitrogen™ DNA Polymerase I, 250 Units

Invitrogen™ DNA Polymerase I, 250 Units

DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.

Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs

Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Performance and quality testing

Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Contents

• 250 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/µl.