Thermo Scientific™ Exonuclease VII

Thermo Scientific™ Exonuclease VII

Exonuclease VII is a strict single-strand directed enzyme with 5'→3' and 3'→5' exonuclease activities, making it the only bi-directional exonuclease with single-stranded specificity. Exonuclease VII has no apparent requirement for divalent cations, as it is fully active in the presence of EDTA. Initial reaction products are acid insoluble oligonucleotides which are further hydrolyzed into acid soluble form. The products of limited digestions are small oligomers (dimers to dodecamers).