Invitrogen™ Dynabeads™ Antibody Coupling Kit

Invitrogen™ Dynabeads™ Antibody Coupling Kit

Dynabeads® Antibody Coupling Kit allows easy coupling of an antibody of your choice to the surface of uniform, 2.8 µm superparamagnetic Dynabeads® M-270 Epoxy beads. Following immobilization of your antibody, the beads can then be used for experiments such as immunoassays, immunoprecipitation (IP), co-immunoprecipitation (co-IP) of protein complexes, co-IP of protein-nucleic acid complexes, as well as many other downstream applications. The Dynabeads® Epoxy beads in this kit do not contain Tween® detergent, and so are ideal for use in mass spectrometry (MS) analysis. In addition to Dynabeads®, the kit supplies all buffers needed for the coupling and washing steps. Note that the SB buffer supplied in the kit contains Tween® detergent, so the SB buffer will need to be replaced with standard TBS or PBS buffer if the kit is to be used for MS. 

Features

• Optimized buffers and Epoxy-coated Dynabeads® included in the kit
• Easy-to-use protocol with minimal hands-on time
• Covalent antibody coupling to the Dynabeads® avoids co-elution of the antibody with the target protein

Contents

• 60 mg Dynabeads M-270 Epoxy & Buffers required for covalent coupling. Dynabeads™ M-270 Epoxy supplied freeze dried. Multiple buffers provided in various volumes.