Invitrogen™ Dynabeads™ Co-Immunoprecipitation Kit

Invitrogen™ Dynabeads™ Co-Immunoprecipitation Kit

Dynabeads® Co-Immunoprecipitation Kit allows easy coupling of an antibody of your choice to the surface of uniform, 2.8 µm superparamagnetic Dynabeads® M-270 Epoxy beads. Following immobilization of your antibody, use the coupled beads and the buffers supplied in the kit for co-immunoprecipitation (co-IP) of proteins, intact protein complexes, or intact protein-nucleic acid complexes and to elute those complexes from the beads. In addition to Dynabeads®, the kit supplies the buffers needed for efficient antibody coupling, gentle washing, and elution of the co-isolated protein complexes.

Features

• Optimized buffers and Epoxy-coated Dynabeads® for efficient co-IP included in the kit
• Gentle separation technology: preserves native protein conformations and large-protein complexes
• Covalent antibody coupling to the Dynabeads® avoids co-elution of the antibody with the target proteins

Contents

• Dynabeads™ M-270 Epoxy (>60 mg freeze-dried)
• Buffer C1 (20 mL)
• Buffer C2 (8 mL)
• Buffer HB (15 mL)
• Buffer LB (15 mL)
• Buffer SB (40 mL)
• 5X IP Buffer (120 mL)
• 5X LWB (24 mL)
• Buffer EB (4 mL)