Invitrogen™ Dynabeads™ CD15

Invitrogen™ Dynabeads™ CD15

Dynabeads™ CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads™ CD15 can also be used to positively isolate CD15+ cells for molecular applications.

About Dynabeads™ CD15, Dynabeads™ CD15 are uniform, superparamagnetic polystyrene beads (4.5 µm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.

Magnetic bead-;based separation offers easy handling, Dynabeads™ CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.

Features

• Efficient depletion of granulocytes (neutrophils and eosinophils)
• Isolation of granulocytes directly from whole blood for molecular applications
• No columns required

Contents

• 5 ml Dynabeads™ CD15 coated with anti-CD15 monoclonal antibody.