Invitrogen™ Dynabeads™ CD14
On demandInvitrogen™ Dynabeads™ CD14
Dynabeads™ CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests.
About Dynabeads™ CD14, Dynabeads™ CD14 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD14 membrane antigen mainly expressed mainly on human monocytes. Because of the bead size, Dynabeads™ CD14 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads™ CD14 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified.
Magnetic bead–based separation offers easy handling, Dynabeads™ CD14 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.
Features
- Fast and efficient isolation of CD14+ cells from any sample—no columns required
- High purity, yield, and viability of positively isolated CD14+ cells for molecular downstream assays
- Fast and efficient depletion of CD14+ cells from any sample
Contents
- 5 ml Dynabeads™ CD14 coated with anti-CD14 monoclonal antibody.
There are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/8b6f2684-3ffd-471b-ba90-37b3d5a9e953.png)
Thermo Scientific™ COUNT-CAL™ Count Precision Size Standards, 70 μm
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/09828e59-db39-4f7f-98cf-d1af290f7765.png)
Thermo Scientific™ Dyed Red Aqueous Fluorescent Particles, 2.0 μm
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/30d616bd-5c79-48b0-8fea-81302d6183bd.png)
Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, 4 μm, 1 mL, Level 12
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/d74ed607-9364-46be-98e6-5c8279e922f9.png)
Thermo Scientific™ 3000 Series Nanosphere™ Size Standards, 600 nm
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/b06be4a2-9fa3-4f51-963e-e826dc7b5c33.png)
Thermo Scientific™ 5000 Series Polymer Particle Suspensions, 1.5 μm, 15 mL
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/051ee1ba-a6a7-4a31-a181-527f3716b069.png)
Invitrogen™ FluoSpheres™ Polystyrene Microspheres, 15 µm, Red-Orange Fluorescent (565/580), For Blood Flow Determination
$ 856