Invitrogen™ Dynabeads™ CD14

Invitrogen™ Dynabeads™ CD14

Dynabeads™ CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests.

About Dynabeads™ CD14, Dynabeads™ CD14 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD14 membrane antigen mainly expressed mainly on human monocytes. Because of the bead size, Dynabeads™ CD14 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads™ CD14 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified.

Magnetic bead–based separation offers easy handling, Dynabeads™ CD14 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Features

• Fast and efficient isolation of CD14⁺ cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD14⁺ cells for molecular downstream assays
• Fast and efficient depletion of CD14⁺ cells from any sample

Contents

• 5 ml Dynabeads™ CD14 coated with anti-CD14 monoclonal antibody.