Invitrogen™ Dynabeads™ CD19 Pan B

Invitrogen™ Dynabeads™ CD19 Pan B

Dynabeads™ CD19 Pan B are superparamagnetic beads covalently coupled with an anti-human CD19 antibody that enable easy isolation or depletion of human CD19⁺ B cells directly from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests.

About Dynabeads™ CD19 Pan B, Dynabeads™ CD19 pan B are uniform (4.5 µm diameter) superparamagnetic beads coated with a primary monoclonal antibody specific for the CD19 membrane antigen mainly expressed on human B cells. Because of the bead size, Dynabeads™ CD19 pan B can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads™ CD19 pan B will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD19+ cells for cell-based applications, or you need to check your samples with flow cytometry, you can combine with DETACHaBEAD™ CD19 (purchased separately to get bead- and antibody-free cells) or use Dynabeads™ Untouched™ Human B Cells to obtain untouched human B cells.

Magnetic bead-based separation offers easy handling, Dynabeads™ CD19 Pan B is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Features

• Fast and efficient isolation of CD19⁺ B cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD19⁺ cells for molecular downstream assays or efficient depletion of CD19⁺ B cells
• Fast and efficient depletion of CD19⁺ T cells from any sample

Contents

• 5 ml Dynabeads™ CD19 (Pan B) coated with anti-CD19 monoclonal antibody.