Invitrogen™ Dynabeads™ Untouched™ Human B Cells Kit

Invitrogen™ Dynabeads™ Untouched™ Human B Cells Kit

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.

Features

• High purity, recovery and viability of human untouched B cells
• Easy to use and to scale up
• No columns required

Contents

• 10 ml Depletion MyOne™ SA Dynabeads™ and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A).