Invitrogen™ Dynabeads™ CD4
On demandInvitrogen™ Dynabeads™ CD4
Dynabeads™ CD4 are superparamagnetic beads coupled with an anti-human CD4 antibody that enable easy isolation or deplettion of human CD4+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests.
About Dynabeads™ CD4, Dynabeads™ CD4 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on human T helper cells. Because of the bead size, Dynabeads™ CD4 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads™ CD4 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified.
Magnetic bead–based separation offers easy handling, Dynabeads™ CD4 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.
Features
- Fast and efficient isolation of CD4+ T cells from any sample—no columns required
- High purity, yield, and viability of positively isolated CD4+ T cells for molecular downstream assays
- Fast and efficient depletion of CD4+ T cells from any sample
Contents
- 5 ml Dynabeads™ CD4 coated with anti-CD4 monoclonal antibody.
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