Invitrogen™ Dynabeads™ CD8

Invitrogen™ Dynabeads™ CD8

Dynabeads™ CD8 are superparamagnetic beads coupled with an anti-human CD8 antibody that enable easy isolation or depletion of human CD8+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests.

About Dynabeads™ CD8, Dynabeads™ CD8 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD8 membrane antigen mainly expressed on human cytotoxic T cells. Because of the bead size, Dynabeads™ CD8 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads™ CD8 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified.

Magnetic bead–based separation offers easy handling, Dynabeads™ CD8 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Features

• Fast and efficient isolation of CD8⁺ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD8⁺ T cells for molecular downstream assays
• Fast and efficient depletion of CD8⁺ T cells from any sample

Contents

• 5 ml Dynabeads™ CD8 coated with anti-CD8 monoclonal antibody.