Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506/780 Sample Pack
On demandInvitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506/780 Sample Pack
Fixable
Viability Dye eFluor™ 506 and 780 are viability dyes that can be used
to irreversibly label dead cells prior to cryopreservation, fixation
and/or permeabilization procedures. Unlike 7-AAD and propidium iodide,
cells labeled with Fixable Viability Dyes can be washed, fixed,
permabilized, and stained for intracellular antigens without any loss of
staining intensity of the dead cells. Thus, using Fixable Viability
Dyes allows dead cells to be excluded from analysis when intracellular
targets are being studied. Fixable Viability Dyes may be used to label
cells from all species.
Fixable
Viability Dye eFluor™ 506 can be excited by the violet (405 nm) laser
line and has a peak emission of 506 nm that can be detected using a
510/50 band pass filter (equivalent to AmCyan). Fixable Viability Dye
eFluor™ 780 can be excited by the red (633 nm) laser line and has a peak
emission of 780 nm that can be detected using a 780/60 band pass filter
(equivalent to APC-eFluor™ 780 or APC-Alexa Fluor™ 750). Please make
sure that your instrument is capable of detecting these dyes. For
compensation, it is recommended to use a sample of the cells of interest
stained with the Fixable Viability Dye. If the percentage of dead cells
is expected to be less than 5%, then it is recommended to take a small
aliquot of cells and heat them at 65°C for 1 minute then immediately
place on ice for 1 minute. After this treatment, the heat-killed cells
can be combined 1:1 with live cells and then stained with the Fixable
Viability Dye.
Fixable
Viability Dye eFluor™ 506 and 780 are supplied as a pre-diluted
solutions prepared in high-quality, anhydrous DMSO. They should be
protected from light and moisture. Store at less than or equal to -70°C
with dessicant. They may be freeze-thawed up to 20 times. Allow vials to
equilibrate to room temperature before opening.
Contents
- Fixable Viability Dye eFluor® 506: 100 tests - 1 vial
- Fixable Viability Dye eFluor® 780: 100 tests - 1 vial
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