Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry eFluor™ 450

Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry eFluor™ 450

The eBioscience BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.

Contents

• BrdU: 5 x 1 mL sterile vials; store at less than or equal to -70°C.
• DNase I: 10 x 0.3 mL vials; store at less than or equal to -70°C.
• Anti-BrdU eFluor® 450 Antibody (clone BU20A): 1 x 100 test vial; store at 2-8°C.
• BrdU Staining Buffer Concentrate (4X): 1 x 30 mL bottle; store at 2-8°C.
• Fixation/Permeabilization Diluent: 1 x 100 mL bottle; store at 2-8°C.