Invitrogen EGF Human ELISA Kit, 2 x 96 Tests

Invitrogen EGF Human ELISA Kit, 2 x 96 Tests

The Human Epidermal Growth Factor (EGF) ELISA quantitates Hu EGF in human serum, plasma, buffered solution, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EGF.

Principle of the method

The Human EGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 5.3%
• Intraassay CV: 4.4%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents    

• Hu EGF Standard, lyophilized; contains 0.1% sodium azide. Refer to vial label for quantity and reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Plate, 96-well strip-well plate, 1 plate
• Hu EGF Biotin Conjugate; contains 0.1% sodium azide, 11 mL
• Streptavidin-HRP (100X), 0.15 mL
• Streptavidin-HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3