Invitrogen p38 MAPK (Total) Human ELISA Kit, 96 Tests

Invitrogen p38 MAPK (Total) Human ELISA Kit, 96 Tests

The Human p38 MAPK (Total) ELISA quantitates Hu p38 MAPK (Total) in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu p38 MAPK (Total).

Principle of the method
The Human p38 MAPK (Total) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C
• Interassay CV: 7.3%
• Intraassay CV: 5.1%

Contents

• p38 MAPK (Total) Standard, lyophilized; contains 0.1% sodium azide. Refer to vial label for quantity and reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Plate, 96-well strip-well plate, 1 plate
• p38 MAPK (Total) Detection Antibody; contains 0.1% sodium azide, 11 mL
• Anti‑Rabbit IgG HRP (100X); contains 3.3 mM thymol, 0.125 mL
• HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3