Invitrogen Endothelin-1 (ET-1) Human ELISA Kit, 10 x 96 Tests

Invitrogen Endothelin-1 (ET-1) Human ELISA Kit, 10 x 96 Tests

The Endothelin-1 (ET-1) Multispecies ELISA quantitates Endothelin-1 in human, bovine, procine, dog, rat, and mouse serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Endothelin-1.

Principle of the method

The Endothelin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 3.7%
• Intraassay CV: 3.3%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Endothelin-1 Standard; 10 ng/mL endothelin-1, 50 μL
• Assay Buffer Concentrate (5X), 28 mL
• Endothelin-1 Antibody Coated Wells, 96-well strip-well plate, 1 plate
• Endothelin-1 Conjugate, 5 mL
• Extraction Solution, 50 mL
• Wash Buffer Concentrate (20X), 30 mL
• Tetramethylbenzidine (TMB) Substrate, 11 mL
• Stop Solution; contains 1 M HCl, CAUSTIC, 5 mL
• Plate Sealer, 2