Invitrogen™ EnzChek™ Paraoxonase Assay Kit

Invitrogen™ EnzChek™ Paraoxonase Assay Kit

The EnzChek™ Paraoxonase Assay Kit is a highly sensitive, homogenous fluorometric assay for the organophosphatase acitivty of paraoxonase. Based on the hydrolysis of a fluorogenic analog, this assay is >10-fold more sensitive than the colorimetric assay, and unlike the colorimetric assay, can distinguish samples of very similar paraoxonase activity. Under standard conditions, the assay requires only 5 µl of serum, yields a signal in as little as 15 minutes, and is linear for up to 50 minutes. The assay requires only a single reaction that can be either continuously monitored or terminated using the stop reagent included in the kit.

Features

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria
• For Use With (Equipment): Microplate Reader