Invitrogen ERK1/ERK2 (Phospho) [pT202/pY204]/[pT185/pY187] Multispecies InstantOne™ ELISA Kit, 96 Tests

Invitrogen ERK1/ERK2 (Phospho) [pT202/pY204]/[pT185/pY187] Multispecies InstantOne™ ELISA Kit, 96 Tests

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster ERK 1/2 in cell lysates. Detection of rat ERK 1/2 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Features

• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• User Manual
• phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Capture Antibody Reagent (Part No. IOPERKCAR1) - 3 mL
• phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Detection Antibody Reagent (Part No. IOPERKDAR1) - 3 mL
• InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed)
• Cell Lysis Buffer (5X) (Part No. IOCLB1) - 12 mL
• Enhancer Solution (Part No. IOES1) - 1 mL
• Wash Buffer (10X) (Part No. IOWB1) - 15 mL
• Detection Reagent (TMB Substrate) (Part No. IODR1) - 12 mL
• Stop Solution (Part No. IOSS1) - 12 mL
• Positive Control Cell Lysate (lyophilized) (Part No. IOERKPC1) – 0.25 mL
• Plate seal - 1 ea