Invitrogen IgG1 Human ELISA Kit Biotinylated Detection Antibody, 96 Tests
On demandInvitrogen IgG1 Human ELISA Kit Biotinylated Detection Antibody, 96 Tests
The Human Immunoglobulin G1 (Hu IgG1) ELISA quantitates Hu IgG1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IgG1.
Principle of the method
The Human IgG1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Assay Diluent concentrate
- Biotinylated Detection Antibody
- SAV-HRP
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers
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