Invitrogen Estradiol Human ELISA Kit, 96 Tests

Invitrogen Estradiol Human ELISA Kit, 96 Tests

The Human Estradiol ELISA quantitates Hu 17-beta Estradiol in human serum and plasma. The assay will exclusively recognize both natural and recombinant Hu Estradiol.

Principle of the method

The Human Estradiol solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 6.1%
• Intraassay CV: 4.3%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents    

• Standard 0 pg/mL in human serum, with preservatives. Lyophilized. Refer to vial label for quantity and reconstitution volume. 1 vial 
• Standards 1 - 5 in human serum, with preservatives. Lyophilized. Refer to vial label for concentration and reconstitution volume. 5 vials 
• Controls 1 and 2 in human serum, with preservatives. Lyophilized. Refer to vial label for reconstitution volume and range. 2 vials 
• Anti-Rabbit IgG-Coated Wells, 96 wells per plate. 1 plate 
• Anti-Estradiol; lyophilized. Refer to vial label for reconstitution volume. 1 vial 
• Estradiol-HRP Conjugate Concentrate. Contains phospate buffer with preservatives; 0.5 ml per vial. 1 vial 
• Conjugate Buffer for dilution of Estradiol-HRP conjugate; 6 mL per vial. 3 vials 
• Wash Buffer Concentrate (200x). 10 mL per bottle. 1 bottle 
• Concentrated Chromogen, Tetramethylbenzidine (TMB) in DMF, 1 mL per vial. 1 vial 
• Substrate Buffer: H2 O 2 in acetate/citrate buffer; 21 mL per bottle. 3 bottles 
• Stop Solution; 1.8N H2 SO 4 ; 6 mL per bottle. 1 bottle