Invitrogen GM-CSF Human Uncoated ELISA Kit, 10 x 96 Tests
On demandInvitrogen GM-CSF Human Uncoated ELISA Kit, 10 x 96 Tests
The Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for
performing quantitative enzyme linked immunosorbent assays (ELISA) to
detect and quantify protein levels of human GM-CSF. Wash Buffer and Stop
Solution are needed to complete the ELISA reaction and are sold
separately.
Principle of the method
ELISAs
are designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody is coated to the
bottom of the wells of a microplate, which is an overnight process.
Samples, standards, or controls are then added into these wells and bind
to the immobilized (capture) antibody. A sandwich is formed by the
addition of the second (detector) antibody, a substrate solution is
added that reacts with the enzyme-antibody-target complex to produce
measurable signal. The intensity of this signal is directly proportional
to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Storage: 2-8°C
Contents
- Capture Antibody: Pre-titrated, purified antibody
- Detection Antibody: Pre-titrated, biotin-conjugated antibody
- Standard: Recombinant protein for generating standard curve and calibrating samples
- 10X Coating Buffer: Buffer for plating the Capture Antibody
- 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
- Enzyme: Pre-titrated Avidin-HRP
- Substrate: 1X TMB Solution
- Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
- 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -76, -86)
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