Invitrogen Estriol ELISA Kit, 10 x 96 Tests

Invitrogen Estriol ELISA Kit, 10 x 96 Tests

The Estriol Competitive ELISA quantitates estriol in serum, plasma, urine, saliva, dried fecal extracts or or cell culture medium.|Principle of the method: The Estriol Competitive ELISA research-use-only kit is designed to quantitatively measure estriol independent of species. An estriol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit estriol antibodies and estriol-peroxidase conjugate is added to the wells. The estriol-peroxidase conjugate and any estriol in the sample will compete to bind to the anti-rabbit estriol antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound estriol-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of estriol present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Features  

• Homogenous (no wash): No    
• Interassay CV: 10%%
• Intraassay CV: 12%%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Coated Clear 96-well plate; strip-well plate coated with goat anti-rabbit IgG, 1 plate
• Estriol Standard; 120,000 pg/mL, 125 μL
• Estriol Antibody, 3 mL
• Estriol Conjugate, 3 mL
• Assay Buffer Concentrate (5X), 28 mL
• Wash Buffer Concentrate (20X), 30 mL
• TMB (Tetramethylbenzidine) Substrate, 11 mL
• Stop Solution; contains 1 M HCl, CAUSITC, 5 mL
• Plate Sealer, 1