Invitrogen Estrone Competitive ELISA Kit, 96 Tests

Invitrogen Estrone Competitive ELISA Kit, 96 Tests

The Multispecies Estrone ELISA quantitates estrone in fecal extracts, urine, and tissue culture media.

Principle of the method

The Estrone Competitive ELISA research-use-only kit is designed to quantitatively measure estrone present in extracted dried fecal samples, urine, and tissue culture media samples. The kit is unique as it measures both non-conjugated estrone, estrone-3-sulfate, and estrone 3-glucuronide in urine and fecal samples with almost equal affinity, allowing for non-invasive testing of this steroid. An estrone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An estrone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estrone to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estrone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Features  

• Homogenous (no wash): No    
• Interassay CV: 5.5%
• Intraassay CV: 4.7%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Estrone Standard; 20,000 pg/mL estrone in a special stabilizi ng solution, 125 μL
• Assay Buffer Concentrate (5X), 28 mL
• Antibody Coated Wells, 96-well strip-well plate coated with goat anti-rabbit IgG, 1 plate
• Estrone Antibody, 3 mL
• Estrone Conjugate, 3 mL
• Wash Buffer Concentrate (20X), 30 mL
• TMB (Tetramethylbenzidi ne) Substrate, 11 mL
• Stop Solution; contains 1 M HCl, CAUSTIC, 5 mL
• Plate Sealer, 1