Invitrogen Estrone Competitive ELISA Kit, 10 x 96 Tests

Invitrogen Estrone Competitive ELISA Kit, 10 x 96 Tests

The Multispecies Estrone ELISA quantitates estrone in fecal extracts, urine, and tissue culture media.

Principle of the method

The Estrone Competitive ELISA research-use-only kit is designed to quantitatively measure estrone present in extracted dried fecal samples, urine, and tissue culture media samples. The kit is unique as it measures both non-conjugated estrone, estrone-3-sulfate, and estrone 3-glucuronide in urine and fecal samples with almost equal affinity, allowing for non-invasive testing of this steroid. An estrone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An estrone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estrone to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estrone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Features  

• Homogenous (no wash): No    
• Interassay CV: 5.5%
• Intraassay CV: 4.7%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Estrone Standard; 20,000 pg/mL estrone in a special stabilizi ng solution, 125 μL
• Assay Buffer Concentrate (5X), 28 mL
• Antibody Coated Wells, 96-well strip-well plate coated with goat anti-rabbit IgG, 1 plate
• Estrone Antibody, 3 mL
• Estrone Conjugate, 3 mL
• Wash Buffer Concentrate (20X), 30 mL
• TMB (Tetramethylbenzidi ne) Substrate, 11 mL
• Stop Solution; contains 1 M HCl, CAUSTIC, 5 mL
• Plate Sealer, 1