Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit Green Features, 400 Reactions

Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit Green Features, 400 Reactions

Ambion® Fast SYBR® Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. The kit includes sufficient reagents for 400 reactions. The Fast SYBR® Green Cells-to-CT™ Kit contains AmpliTaq® Fast DNA Polymerase, UP, which allows instant hot start, thus minimizing non-specific product formation with fast real-time PCR.

Features

• Includes optimized reagents to work efficiently, right out of the box
• Simple, effective Cells-to-CT™ technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
• Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Detection Method: SYBR
• Green Features: Fewer resources used and less waste, Less hazardous
• Hot Start: Built-In Hot Start
• Label or Dye: SYBR™ Green
• Passive Reference Dye: ROX (Pre-mixed)
• Polymerase: AmpliTaq™ Fast DNA Polymerase
• Primer Probe Compatibility: Unlabeled Oligos
• Purity: UP (Ultra Pure)
• Reverse Transcriptase: M-MLV
• Shipping Condition: Dry Ice
• Sample Type: Cells

Contents

• Stop Solution: 2 x 1 mL
• DNase I: 220 μL
• 20X RT Enzyme Mix: 1.1 mL
• Lysis Solution: 22 mL
• 2X SYBR® RT Buffer: 22 mL
• Fast SYBR® Green PCR Master Mix: 4 x 5 mL
• Nuclease-free Water: 50 mL