Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments, 100 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
100 Reactions
Order number :
11754100
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments, 100 Reactions
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Features
- Efficiency—Offers significant time and cost savings for increased throughput
- Convenience—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
- Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
- Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
- Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
- For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex
Contents
- Resuspension Buffer: 10 mL
- Lysis Enhancer: 1 mL
- DNase I, Amplification Grade (1 U/µl): 500 µL
- 10X DNase I Buffer: 160 µL
- 25 mM EDTA: 400 µL
- SuperScript™ III RT/Platinum™ Taq Mix: 100 µL
- 2X Reaction Mix with ROX™: 2 X 1.25 mL
- 50mM MgSO4: 1 mL
- DEPC-treated water: 2 mL
- HeLa Total RNA (10 ng/µl): 10 µL
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