Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments, 100 Reactions

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments, 100 Reactions

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features

• Efficiency—Offers significant time and cost savings for increased throughput
• Convenience—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
• Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
• Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex

Contents

• Resuspension Buffer:  10 mL
• Lysis Enhancer:  1 mL
• DNase I, Amplification Grade (1 U/µl):  500 µL
• 10X DNase I Buffer:  160 µL
• 25 mM EDTA:  400 µL
• SuperScript™ III RT/Platinum™ Taq Mix:  100 µL
• 2X Reaction Mix with ROX™:  2 X 1.25 mL
• 50mM MgSO4:  1 mL  
• DEPC-treated water:  2 mL
• HeLa Total RNA (10 ng/µl):  10 µL