Invitrogen Fc Receptor Binding Inhibitor Polyclonal Antibody, 100 Tests, eBioscience™

Invitrogen Fc Receptor Binding Inhibitor Polyclonal Antibody, 100 Tests, eBioscience™

The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs.

Applications: Functional Assay (FN).

Features  

• Purity: Greater than 90%, as determined by SDS-PAGE.
• Aggregation: Less than 10%, as determined by HPLC.
• Filtration: 0.2 µm post-manufacturing filtered.