Invitrogen™ FGF2 Human ELISA Kit, 96 Tests

 Invitrogen™ FGF2 Human ELISA Kit, 96 Tests

The Human FGF-b ELISA Kit quantifies human Fibroblast Growth Factor basic (FGF-b) in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18 kDa isoform and recombinant FGF basic-146 and FGF basic-157.

Principle of the method

The Human FGF-b solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Interassay CV: 5.5%
• Intraassay CV: 4.3%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Hu FGF-b Standard, recombinant Hu FGF-b-146 expressed in E. coli; contains 0.1% sodium azide, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Wells, 96-well plate, 1 plate
• Hu FGF-b Biotin Conjugate; contains 0.1% sodium azide, 11 mL
• Streptavidin-HRP (100X); contains 3.3 mM thymol, 0.125 mL
• Streptavidin-HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Adhesive Plate Covers, 3