Invitrogen Fibronectin Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen Fibronectin Human ELISA Kit, 10 x 96 Tests
The Human Fibronectin ELISA quantitates Hu Fibronectin in human serum,
plasma, urine or cell culture medium. The assay will exclusively
recognize both natural and recombinant Hu Fibronectin.
Principle of the method
The
Human Fibronectin solid-phase sandwich ELISA (enzyme-linked
immunosorbent assay) is designed to measure the amount of the target
bound between a matched antibody pair. A target-specific antibody has
been pre-coated in the wells of the supplied microplate. Samples,
standards, or controls are then added into these wells and bind to the
immobilized (capture) antibody. The sandwich is formed by the addition
of the second (detector) antibody, a substrate solution is added that
reacts with the enzyme-antibody-target complex to produce measurable
signal. The intensity of this signal is directly proportional to the
concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 5.3%
- Intraassay CV: 6.7%
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Sample Diluent
- Assay Buffer concentrate
- Biotinylated Detection Antibody
- SAV-HRP
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers
There are no specifications
There are no report
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