Invitrogen HSPB1 (HSP27) Human ELISA Kit, 5 x 96 Tests

Invitrogen HSPB1 (HSP27) Human ELISA Kit, 5 x 96 Tests

The Human HSPB1 (HSP27) ELISA quantitates Hu HSP27 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSP27.
Principle of the method
The Human HSP27BTC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents    

• Human HSPB1 Antibody Coated wells, 96-well plate, 1 plate
• Human HSPB1 Biotin Conjugate, 2 vials
• Human HSPB1 Standard, recombinant human HSPB1, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent (5X), 15 mL
• Streptavidin-HRP (300X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2