Invitrogen™ Filmtracer™ LIVE/DEAD™ Biofilm Viability Kit
On demand
Order number :
L10316
Invitrogen™ Filmtracer™ LIVE/DEAD™ Biofilm Viability Kit
The
FilmTracer™ LIVE⁄DEAD Biofilm Viability kit provides a two-color
fluorescence assay of bacterial viability, based on membrane integrity,
that has proven usefule for a diverse array of bacterial genera
including those growing in biofilm communities. The LIVE⁄DEAD Biofilm
Viability kit utilizes mixtures of our SYTO® 9 green fluorescent nucleic
acid stain and the red-fluorescent nucleic acid stain, propidium
iodide. These stains differ both in their spectral characteristics and
in their ability to penetrate healthy bacterial cells. When used alone,
the SYTO® 9 stain generally labels all bacteria in a population—those
with intact membranes and those with damaged membranes. In contrast,
propidium iodide penetrates only bacteria with damaged membranes,
causing a rduction in the SYTO® 9 stain fluorescence with both dyes are
present. Thus, with an appropriate mixture of the SYTO™ 9 and propdium
iodide stains, bacteria with intact cell membranes stain fluorescent
green, whereas bacteria with damaged membranes stain fluorescent red
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, Flow Cytometer, Microplate Reader, Fluorescent Imager
Contents
- SYTO® 9 green fluorescent nucleic acid stain (Component A): 482/500 nm
- Propidium iodide (Component B): 490/635 nm
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