Thermo Scientific™ Hoechst 33342 Solution (20 mM)

Thermo Scientific™ Hoechst 33342 Solution (20 mM)

Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques. Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet light and emits blue fluorescence at 460 to 490 nm. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios

Features

• Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells)
• Convenient—provided as an easy-to-use Hoechst dye solution (20 mM)
• Cellular imaging—cell-permeable dye is effective for fixed-cell and live-cell staining
• Counterstain—ideal for use alongside detection of specific targets with fluorescent antibodies for fluorescence microscopy or high-content screening (HCS)
• For Use With (Equipment): Fluorescence Microscope, High Content Instrument
• SubCellular Localization: Nucleus