Invitrogen Flt-3 Ligand Human ProQuantum Immunoassay Kit, 5 X 96 Tests
On demandInvitrogen Flt-3 Ligand Human ProQuantum Immunoassay Kit, 5 X 96 Tests
The ProQuantum Human Flt-3 Immunoassay Kit is designed to provide highly sensitive quantitative measurements of human Flt-3 in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.
ProQuantum immunoassays utilize a matched pair of target-specific
antibodies, each conjugated to a DNA oligonucleotide. During
antibody-analyte binding, the two DNA oligos are brought into close
proximity, which allows for ligation of the two strands and subsequent
creation of a template strand for amplification. This platform leverages
the sensitivity and large dynamic range of Applied Biosystems TaqMan
real-time PCR technology (Figure 1).
The
assay workflow is fast and easy—2 steps in 2 hours. There are a total
of 7 components in each kit (Figure 2). First, mix the
antibody-conjugates, dilute the sample, and create the standard curve in
a working plate. Then, using a multi-channel pipette, add the
antibody-conjugates and sample (or standard) into the wells of a PCR
plate and incubate for 1 hour. Combine the master mix and ligase and add
to the wells of the PCR plate, then run the plate on any qPCR
instrument. After the run is complete, import the results file into the
ProQuantum cloud-based software at
https://apps.thermofisher.com/apps/proquantum. Using this software, the
data can be analyzed easily to obtain protein concentration values. The
software allows you to set up standard curves, design plate layouts, set
up customized assay instructions, and obtain robust statistical
group-wise comparisons.
Features
- High sensitivity—detect low levels of protein with greater sensitivity than traditional methods
- Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
- Small sample consumption—use 2–5 µL of sample (compared to 75 µL for triplicate wells with other methods)
- Fast, easy workflow—2 hours from sample to answer, with no wash steps
- No proprietary instrument to purchase—runs on any real-time PCR instrument
- Homogenous (no wash): Yes
- Shipping condition: Dry Ice
- Storage: -20°C
Contents
- Human Flt-3 Ligand Antibody-conjugate A, 15 μL
- Human Flt-3 Ligand Antibody-conjugate B, 15 μL
- Human Flt-3 Ligand Protein Standard, lyophilized, 2 vials
- Master Mix, 5 mL
- Ligase, 40 μL
- Antibody-conjugate Dilution Buffer, 700 μL
- Assay Dilution Buffer, 7.5 mL
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