Invitrogen Human Flt-3L ELISA Kit, 96 Tests
On demandInvitrogen Human Flt-3L ELISA Kit, 96 Tests
The Human FLT-3Ligand (Hu Flt-3L) ELISA quantitates Hu Flt-3L in human
serum, plasma, or cell culture medium. The assay will exclusively
recognize both natural and recombinant Hu Flt-3L.
Principle of the method
The
Human Flt-3L solid-phase sandwich ELISA (enzyme-linked immunosorbent
assay) is designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody has been pre-coated in
the wells of the supplied microplate. Samples, standards, or controls
are then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Storage: 2-8°C
- Shipping condition: Wet or Dry Ice
Contents
- Human FLT-3 ligand Antibody Coated wells, 96-well plate, 1 plate
- Human FLT-3 ligand Biotin Conjugate, 2 vials
- Human FLT-3 ligand Standard, recombinant human Flt-3, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (600X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
There are no specifications
There are no report
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