Invitrogen™ Fluo-4 Calcium Imaging Kit

Invitrogen™ Fluo-4 Calcium Imaging Kit

The Fluo-4 Calcium Imaging Kit has been designed for the specific detection of calcium flux by imaging applications. Upon binding of calcium, a large increase (>100-fold) in fluorescence emission can be detected. To minimize time to experimental results, this kit contains all of the reagents necessary for the detection of calcium flux from live cells.

Features

• Emission from the calcium-bound Fluo-4 dye (ex/em 494/506 nm) can be detected with standard FITC filters
• Upon binding calcium, fluorescence intensity increases >100-fold
• K_d for Ca²⁺ in buffer: ∼335 nM
• Contains all necessary reagents, reducing time to experimental results
• Exhibits fluorescence increase upon binding calcium with little shift in wavelength

Contents

• 50 µL of Fluo-4 AM Dye in DMSO
• 500 µL of PowerLoad™ Concentrate
• 5 mL of Neuro Background Suppressor
• 1 vial of Probenecid