Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells
On demand
Order number :
T1030
Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells
The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.
For use with: Luminometer (Microplate)
Features
- Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
- The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
- Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
- Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range.
- Assay sensitivity is 100 to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies.
- Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
- Assay can be completed in about one hour, providing fast assay turnaround.
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