Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System, 400 Assays

Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System, 400 Assays

Phospha-Light™ assay system is a chemiluminescent reporter gene assay for the sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein. 

For use with: Luminometer (Microplate)

Features

• Detection of a secreted reporter permits analysis without destroying valuable cells.
• Wide dynamic range of alkaline phosphatase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for b-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without the need to perform numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround. 

Contents

• This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase.