Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells, 1800 Assays

Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells, 1800 Assays

The Galacto-Star™ One-Step β-galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.

For Use With (Equipment): Luminometer (Microplate)

Features

• Glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.

• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.

• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.

• Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.

• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.

• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.

• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive

The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Versatile

The Galacto-Star™ system includes Galacton-Star® chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.

Long-Lasting Signal

Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star® substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter. 

• Compatible Cells: Mammalian Cells
• High-throughput Compatibility: High-throughput Compatible
• Product Line: Galacto-Star™, NovaBright™
• Readout: End Point
• Sample Type: Cell Cultures
• Substrate: Galacton-Star™
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase
• Technique: Enhanced Chemiluminescence
• Form: Liquid
• Format: 96-well plate
• Label Type: Unlabeled
• Product Type: β-Galactosidase Reporter Gene Assay System

Contents

• Microplate assays per kit 
• Lysis Solution : 00 mM potassium phosphate (pH 7.8), 0.2% Triton X-100 (for mammalian cells, in T1012, T1014, T1013)
• OR 5X Z Buffer: 0.5 M sodium phosphate (pH 7.1), 50 mM KCl, 5 mM MgSO4 (for yeast cells, in T1019, T1020). 
• Galacton-Star substrate: 50X concentrate 
• Reaction Buffer Diluent: 100 mM sodium phosphate (pH 7.5), 1 mM magnesium chloride, 5% Sapphire-II enhancer.