Thermo Scientific™ Pierce™ Coomassie Plus (Bradford) Assay Kit

Thermo Scientific™ Pierce™ Coomassie Plus (Bradford) Assay Kit

The Pierce Coomassie Plus (Bradford) Protein Assay is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent to quickly measure total protein concentration compared to a protein standard. The Pierce Coomassie Plus Assay Reagent provides increased linearity and half the protein-to-protein variability of other commercial Bradford assay formulations.

The assay is performed at room temperature. Simply add the sample to the tube containing reagent and the resultant blue color is measured at 595 nm following a short room-temperature incubation. The protein assay is compatible with most salts, solvents, buffers, thiols, reducing substances, and metal-chelating agents encountered in protein samples. The assay can be performed in either test tube or microplate format.

Features

• Colorimetric—measure with a standard spectrophotometer or plate reader at 595 nm
• Easy to use—single reagent; no working reagent preparation required
• Fast—almost immediate color development; add, mix, and read results
• Assay range—detects protein concentration in the range 1 to 1500 µg/mL
• Better—improved linearity and response uniformity compared to traditional Bradford formulations
• Flexible—microplate and cuvette protocols provided and adaptable to several target working ranges

Contents

• Pierce Coomassie Plus Assay Reagent, 950 mL
• Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL