Invitrogen GAS6 Mouse ELISA Kit, 5 x 96 Tests

Invitrogen GAS6 Mouse ELISA Kit, 5 x 96 Tests

The Mouse GAS6 ELISA quantitates Ms GAS6 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms GAS6.

Principle of the method

The Mouse GAS6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen

Features 

• Interassay CV: <12%
• Intraassay CV: <10%
• Homogenous (no wash): No
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Mouse GAS6 Antibody Coated wells, 96-well plate, 1 plate
• Mouse GAS6 Biotin Conjugate, 2 vials
• Mouse GAS6 Standard, recombinant mouse GAS6, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 30 mL
• Assay Diluent C, 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (300X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2