Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells, is a complete kit that includes master mix, positive control, water, and One Shot TOP10 chemically competent E. coli.

Features

Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction

Efficient—robust efficiency provides successful clones for both simple and challenging constructs

Flexible—design guidelines allow assembly into any vector of your choice

Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells

Trusted—over 4000 citations in the scientific literature highlighting great success

Contents

Master mix

Positive control

Water

One Shot TOP10 chemically competent E. coli