Invitrogen™ pGlow TOPO™ TA Expression Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
20 reactions
Order number :
K483001
Invitrogen™ pGlow TOPO™ TA Expression Kit
The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity. Cycle 3 GFP is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.
Contents
- pGlow-TOPO® : 20 μL
- 10X PCR Buffer: 100 μL
- dNTP Mix: 10 μL
- Salt Solution: 50
- T7 Sequencing Primer: 20
- LacZ Reverse or GFP Reverse Sequencing Primer: 20
- Control PCR Template: 10
- Control PCR Primers: 10
- Sterile Water: 1 mL
- SOC Medium: 6 mL
- TOP10 cells: 21 x 50 μL
- pUC19 Control DNA: 50 μL
- T7 Primer: 328 pmoles
- LacZ Reverse Primer: 350 pmoles
- GFP Reverse Primer: 296 pmoles
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