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Invitrogen™ pGlow TOPO™ TA Expression Kit

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 20 reactions
Order number : K483001
(Ex-work price)
$ On demand

Invitrogen™ pGlow TOPO™ TA Expression Kit

 

The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity. Cycle 3 GFP is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.


Contents

  • pGlow-TOPO® : 20 μL
  • 10X PCR Buffer: 100 μL
  • dNTP Mix: 10 μL
  • Salt Solution: 50
  • T7 Sequencing Primer: 20
  • LacZ Reverse or GFP Reverse Sequencing Primer: 20
  • Control PCR Template: 10
  • Control PCR Primers: 10
  • Sterile Water: 1 mL
  • SOC Medium: 6 mL
  • TOP10 cells: 21 x 50 μL
  • pUC19 Control DNA: 50 μL
  • T7 Primer: 328 pmoles
  • LacZ Reverse Primer: 350 pmoles
  • GFP Reverse Primer: 296 pmoles
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