Invitrogen™ GeneArt™ Seamless PLUS Cloning and Assembly Kit

Invitrogen™ GeneArt™ Seamless PLUS Cloning and Assembly Kit

Like our first generation Seamless Cloning and Assembly Kit, GeneArt® Seamless PLUS Cloning and Assembly Kit is the complete kit for simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute or less, room temperature reaction. However, Seamless PLUS offers several advantages over previous kits:

Features

• Increased Efficiency: pre-cloning option for large fragments for increased cloning efficiency
• Larger Constructs: create constructs up to 40 kb
• Versatility: high capacity, broad-range conjugative vector that replicates in most Gram negative bacteria

Contents

• GeneArt Enzyme Mix (2X)
• pUC19L Linearized Vector
• pYES7L Linearized Vector
• 2 control inserts
• One Shot™ DH10Bâ„¢ T1R SA Cells
• pUC19 Control
• Stbl3â„¢/pRK2013 Glycerol Stock
• S.O.C. medium