Invitrogen™ GeneArt™ Linear pUC19L Vector for Seamless Cloning

Invitrogen™ GeneArt™ Linear pUC19L Vector for Seamless Cloning

The GeneArt® Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt® Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.

Features

• lacZα gene allows easy blue⁄white colony screening for selection of recombinants
• pUC origin of replication (high copy vector)
• Ampicillin resistance for selection in E. coli
• 6 convenient and validated restriction sites flanking the cloning region
• Tested for functionality by assembly with 4 DNA fragments using the GeneArt® Seamless Cloning and Assembly Kit

Contents

• 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml.