Invitrogen GITR L/TNFSF18 Human ELISA Kit, 5 x 96 Tests
On demandInvitrogen GITR L/TNFSF18 Human ELISA Kit, 5 x 96 Tests
The Human GITR Ligand/TNFRSF18 (GITR L) ELISA quantitates Hu GITR L in
human serum, plasma, or cell culture medium. The assay will exclusively
recognize both natural and recombinant Hu GITR L.
Principle of the method
The
Human GITR L solid-phase sandwich ELISA (enzyme-linked immunosorbent
assay) is designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody has been pre-coated in
the wells of the supplied microplate. Samples, standards, or controls
are then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Pre-coated 96 well plate
- Standard
- Assay Diluent concentrate
- Biotinylated Detection Antibody
- SAV-HRP
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers